Antibody Production Service

Monoclonal Antibody Production:

MAb production is divided into different phases.
The customer has the option to be invoiced for each phase at its initiation.

Phase I: Immunization(5 mice/antigen)& Sera Testing by ELISA $700
Phase II: Fusion and Colony Screening $1800
Phase III: Subcloning, Colony Expansion & Frozen $350
Phase IV: Isotyping and Ascites/serum free Production (2 clones) $350
Phase V: Antibody Purification by Protein A/G (2 clones, 3mg each) $350
Total Price (Phase I-C V) $3550
Charge for each additional hybridoma $350
Charge for each additional antibody production $350

The customer can provide a ready-to-inject antigen or peptide antigen for MAb production.
When using a peptide supplied by the customer, additional charge for KLH conjugation is $200.

In-vivo Ascites Production:

In-Vivo Production Price per mouse
Ascites Production from 1-25 Mice $25.00
Ascites Production from 26-50 Mice $24.50
Ascites Production from 51-100 Mice $24.00
Ascites Production from 101-150 Mice $23.50
Ascites Production from 151-200 Mice $23.00
Ascites Production from 200+ Mice $19 - $22.50

Polyclonal Antibody Production:

Service Type Service Content Price

Basic Service

Using a ready to inject antigen (2mg) supplied by the customer. Service includes two New Zealand White Rabbits immunization, prebleed, blood collection (40~60ml) and titration. Approx. time required: 3 months $790/antigen
Standard Service

Using a peptide (2mg) supplied by the customer.Service includes peptide conjugation to KLH, two New Zealand White Rabbits immunization, prebleed, blood collection (40~60ml) and titration. Approx. time required: 3 months

$920/antigen
Optional Service Antibody purification by peptide affinity column (peptide supplied by customer, antiserum from 2 rabbits) $450/peptide
Antibody purification by protein A/G $200/antigen

SG could also provide peptide synthesis service which is $16/amono acid.

We also provide additional services to evaluate the application of antibodies on Immunoprecipitation,
Western blotting, and Immunohistochemistry on normal and disease tissues.

Validation of Non-characterized Antibody:

Validation Type Service Content Price
Western analysis (antigen overexpression in mammalian cells) Transient transfection of the target cDNA (provided by customer) into mammalian cells and Western-blot with the antibody. $400
Western analysis (endogenous detection in a mammalian cell line) The customer will choose the cell line or provide the specific cells. $400
Immunoprecipitation analysis (based on antigen overexpression in mammalian cells) Transient transfection of flag -tagged target cDNA (original target cDNA provided by customer) into mammalian cells, immunoprecipitated with target antibody and Western-blot detection with anti-flag. $600
Immunohistochemistry analysis Using a paraffin-embedded normal or tumor tissue selected by the customer: antibody's specification and sensitivity (up to 6 tissue samples). $120/tissue

Examples of Antibody eneration by Shanghai Genomics

Monoclonal antibody against human G1 protein for western blot use

Customer request: To generate monoclonal antibody specifically against human G1 protein which can
be applied in western blot detection, no cross reaction with the other family member proteins (G2 and
G3 protein), but could react with mouse G1 protein.

Antibody Production Service

Polyclonal antibody against human H protein for western blot and immuno-precipitation use

Customer request: To generate polyclonal antibody against human H protein that can be applied in both western
blot detection and immuno-precipitation.

Antibody Production Service

 

 

Contact Info:

Ms. Wei Zhang
Project Management
Office: +86-21-51319016
           +86-21-50802786 Ext: 188
Fax:    +86-21-50802783
Email: service@shanghaigenomics.com
Service Web: www.genomicservice.com

 

 

Shanghai Genomics, Inc.

www.shanghaigenomics.com

647 Song Tao Road, Building 1,Zhangjiang Hi-Tech Park

Shanghai 201203,P.R.China